Date of Award
Master of Science
Jiménez-Ruiz, Francisco Agustín
Brown, Jason L.
Bolek, Matthew G.
Gyrinicola Yamaguti, 1938 are oxyurid mutualists found within the intestinal tract of numerous larval anuran species in Europe, Asia, South America and North America, with 6 species being currently recognized. The systematic placement and hierarchical treatment of the genus has shifted since its discovery, originally considered as its own family (Gyrinicolidae), then treated as a subfamily (Gyrinicolinae) within Cosmocercidae, then considered a member of the Pharyngodonidae, followed by a transference to the Cosmocercidae, and finally a recent proposal to resurrect Gyrinicolidae. The morphology of the uterine tract is a characteristic often used to diagnose members the genus; yet, species show variability in the morphology of these tracts. Until recently very few DNA sequences were available to aid in the phylogenetic placement of this unique group of worms. Within North America, only Gyrinicola batrachiensis is recognized. This species, however, also has a controversial taxonomic history. Historically, two nematodes infecting larval anurans were once recognized in North America: Pharyngodon batrachiensis and Pharyngodon armatus though these were synonymized due to morphological similarity. To evaluate the potential distribution, taxonomic relation, and possible species diversity of Gyrinicola I used an integrative approach utilizing locality, morphology and genetic information of specimens from a wide range within the United States. Phylogenies of the nuclear ribosomal genes 28S, 18S, ITS1-5.8S-ITS2, and newly sequenced partial mitochondrial genomes suggest at least four species groups exist among the nematodes from North America and that these groups, alongside G. japonica, form a well-supported group within Oxyuroidea. This study represents the first genetic evaluation of the diversity of the genus in North America, and the first comparison of these nematodes collected from such distant localities, and clearly indicates that further investigation of these worms is required to characterize their true diversity. It also suggests further representation of Pharyngodonidae from other vertebrate classes may help clarify the relations of this historical grouping to other members of the Oxyuroidea.
Supplementary_Material_2_Male_Proportion_CODE.pdf (321 kB)
Supplementary_Material_3_Female_RAW_CODE.pdf (869 kB)
Supplementary_Material_4_Male_RAW_CODE.pdf (367 kB)
Supplementary_Material_5_Female_Proportion_Results.pdf (1650 kB)
Supplementary_Material_6_Male_Proportion_Results.pdf (1502 kB)
Supplementary_Material_7_Female_RAW_Results.pdf (1900 kB)
Supplementary_Material_8_Male_RAW_Results.pdf (1539 kB)
Supplementary_Material_9_Female_Tukey_Code.pdf (407 kB)
Supplementary_Material_10_Male_Tukey_Code.pdf (207 kB)
Supplementary_Material_11_Female_Tukey_Results.pdf (1372 kB)
Supplementary_Material_12_Male_Tukey_Results.pdf (1212 kB)
28S_Supplementary_Table_I_Fixed.xls (267 kB)
18S_Supplementary_Table_2_Fixed.xls (173 kB)
18S_28S_Supplementary_Table_3_Fixed.xls (238 kB)
Supplementary_Table_IV.xlsx (14 kB)
Supplementary_Table_V.xlsx (11 kB)
Available for download on Thursday, July 18, 2024
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