Date of Award
Doctor of Philosophy
Molecular, Cellular, and Systemic Physiology
The differentiation of growth hormone producing somatotropes in the pituitary gland is dependent upon signaling and transcription factors produced both within and outside the gland. In order to better understand this fundamental process, we have focused on investigating the contribution of forkhead factors FOXO1 and FOXO3. We sought to elucidate whether FOXO1 is sufficient to drive somatotrope differentiation in an over-expression mouse model, identify potential functional redundancy between closely related forkhead family members, and to specify the genetic targets of FOXO1 binding in the pituitary gland. Using a combination of mouse models and molecular techniques we have established a role for Foxo1 in early embryonic development, generated a somatotrope-specific FOXO1 binding enrichment library, revealed functional redundancy between FOXO1 and FOXO3 in the pituitary gland, and discovered a novel protein-protein interaction with YBX1. These results demonstrate FOXO factors are important for pituitary gland formation and function during both pre- and postnatal periods.
This dissertation is only available for download to the SIUC community. Current SIUC affiliates may also access this paper off campus by searching Dissertations & Theses @ Southern Illinois University Carbondale from ProQuest. Others should contact the interlibrary loan department of your local library or contact ProQuest's Dissertation Express service.