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The production of 3-item probe sequences by 20 adults was assessed after the participants were trained to respond to three 3-item sequences (A1→A2→A3, B1→B2→B3, and C1→C2→C3). Each sequence was learned progressively according to the successive phase method (1-item phase, 2-item phase, and then 3-item phase). Performance on the 3 probe sequences was consistent with the formation of classes of mutually substitutable sequence stimuli. Elsewhere, learning of these functional classes was enhanced when subjects were trained with a 5-min compared to a 24-hr intersessions delay. These results were discussed according to experimental conditions and acquisition of learning rules knowledge.