Date of Award


Degree Name

Master of Science


Molecular Biology Microbiology and Biochemistry

First Advisor

Hamilton-Brehm, Scott


Increasingly, microbial ecosystems of the deep continental subsurface are becoming recognized as their own distinct biome. However, access to isolated fractured rock ecosystems is limited to boreholes, mines, and springs. Analysis of these environments is most commonly accomplished through culturing independent methods involving 16S rRNA gene surveys. This provides an understanding of the biodiversity (Bacterial and Archaeal) and implies what functions communities are generally capable of metabolically on a global level. Characterization of the individual microorganisms that inhabit these environments is necessary for a more complete context of how biochemistry and geochemical interactions operate in the oligotrophic conditions of the subsurface.




This thesis is only available for download to the SIUC community. Others should
contact the interlibrary loan department of your local library.