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Abstract

The effects of petroleum products: Petroleum Motor Spirit (petrol, PMS), Automobile Gas Oil (diesel. AGO), Dual Purpose Kero (kerosene, DPK), were investigated on the chlorophyll accumulation in Barbula lambarenensis. This plant with its substrates were collected from its natural populations, taken to the screen house and divided into ten regimes widely separated. Each of these regimes was irrigated with borehole water for two weeks, thrice a week to bring about hydration. The three petroleum products were separately diluted with borehole water in 1:1 ratio to give 50% concentrations. Moreover, 100% of these products were also used .The 50% concentrations were separately used to irrigate the first three regimes, A, B and C for 20 weeks .The D, E and F regimes were irrigated with 100% kerosene, petrol and diesel respectively for the 2 weeks and later with borehole water for twenty weeks while the G regime was irrigated with borehole water (Control). Irrigations were done thrice a week. Samples of B. lambarenensis were collected fortnightly for chlorophyll extraction and the chlorophyll a, b and total chlorophyll content determined with the aid of spectrophotometer .It was observed that the 50% treated moss plants had greater chlorophyll a, b and total chlorophyll than the control but their concentrations were statistically the same. The 100% treatment gave greater chlorophyll a content than the control, PMS treatment had greater chlorophyll b than the control but the reverse were for DPK and AGO treated moss plant. However, all these values were the same statistically. The same results were found for the total chlorophyll except that the DPK, AGO and Control treatments had the same value statistically while DPK, Control and PMS treatments were the same statistically. The 50% treatments had worse effects than the 100% followed by borehole water treatments. Furthermore, the two concentrations had the same effects on the chlorophyll contents but DPK and AGO had significantly greater chlorophyll b concentrations in 50% treated regimes than 100% treatments at 5% level of significance. Petroleum products damaged the chlorophyll apparatus of the plant making the treated to produce greater chlorophyll contents than the Control.

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