Archaea and eukaryotes, in addition to protein-only enzymes, also possess ribonucleoproteins containing an H/ACA guide RNA plus four proteins that produce pseudouridine (Ψ). Although typical conditions for these RNA-guided reactions are known, certain variant conditions allow pseudouridylation. We used mutants of the two stem-loops of the
Majumder, Mrinmoyee, Mukhopadhyay, Shaoni, Kharel, Parinati and Gupta, Ramesh. "The presence of the ACA box in archaeal H/ACA guide RNAs promotes atypical pseudouridylation.." RNA (New York, N.Y.) 26, No. 4 (Apr 2020): 396-418. doi:10.1261/rna.073734.119.