The antimicrobial activities and Minimal Inhibitory Concentration (MIC) of the extracts of Aspilia africana were evaluated against five bacteria (Staphylococcus aureus, Streptococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli). History of the organisms used indicated pathogenicity during laboratory analysis of specimen collected. Leaf of the plant was collected from the farm. The ethanolic and hot aqueous extracts were obtained by standard methods. The antimicrobial activity was conducted using a modified agar well diffusion method. The results showed that the ethanolic extract of Aspilia africana exerted antimicrobial effect on the test organisms at 12.5mg/ml, 25mg/ml, 50mg/ml and 100mg/ml concentrations, while the hot aqueous extract exerted no antibacterial activity against any of the tested organisms at the same concentrations. The ethanolic extract of Aspilia africana showed the highest antibacterial activity with diameter of zone of inhibition of 31.5mm against Pseudomonas aeruginosa, 25.0mm against Klebsiella pneumonia, 21.5mm against Staphylococcus aureus and 17.5mm and least effective against Streptococcus faecalis and Escherichia coli at 100mg/ml concentration. The Minimum Inhibitory concentration (MIC) of the ethanolic extracts was at a concentration 1.325mg/ml for all the organisms. The antibacterial activity of the extract could be enhanced if the components are purified. This plant therefore holds a promise as a potential source of new drug for treating infections caused by these clinical pathogens.
Anibijuwon, I.I.; Duyilemi, O.P.; and Onifade, A.K.
"Antimicrobial Activity of Leaf of Aspila africana on some Pathogenic Organisms of Clinical Origin,"
4, Article 2.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2010/iss4/2