The antibacterial activity of the water and ethanolic extracts of cotton leaf (Gossypium spp.) plus lemon juice (Citrus limon) were tested against pure clinical isolates of Salmonella sp., Shigella sp., E. coli and Klebsiella sp. Cotton leaf is normally used in conjunction with lemon juice by the local populace in Nigeria for the treatment of enteric infections. Decoction method was used for the extraction of the active components from the plant in order to simulate the traditional method of extraction. The Minimum Inhibitory Concentration (MIC) and the diameters of zones of inhibition were determined by broth dilution and Agar diffusion methods respectively. The ethanolic extracts are more effective than the water extracts on the test organisms. The MIC of the water and ethanolic extracts ranged between 1.25 -5.0 w/v on the test organisms. Similarly, the average diameter of zones of inhibition of the water extracts on the test organisms ranged between 3.0 to 13.0mm while that of the ethanolic extracts ranged between 12.0 to 21.0mm. The results of this study showed that E. coli was the most susceptible followed by Klebsiella sp., then Salmonella sp., and finally Shigella sp. at the concentrations used for both water and ethanolic extracts. This observation thereby justifies the traditional uses of these plant extracts among the Nigerian local populace for the treatment of some enteric infections such as dysentery and diarrhoea.
Sule, I. O.; Agbabiaka, T. O.; and Oyeyiola, G. P.
"Tradomedical Values of Cotton Leaf Plus Lemon Juice Against Clinical Bacterial Isolates,"
Ethnobotanical Leaflets: Vol. 2009
, Article 12.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2009/iss4/12