Aerial parts and flowers of Sphaeranthus indicus were extracted with n-hexane, benzene, chloroform, ehtylacetate and acetone. The extracts were screened for their antimicrobial activity using in vitro disc diffusion method at concentrations of 5, 2.5 and 1.25 mg/disc. The Minimum Inhibitory Concentration (MIC) was tested using broth micro dilution method at concentrations ranging from 5 to 0.039 mg/ml. Significant antibacterial and antifungal activity was observed in hexane extract of flower and aerial parts. The flower extract showed MIC as 0.15 mg/ml against Staphylococcus aureus and the highest MIC (5 mg/ml) was noted for S. epidermidis. The n-hexane extracts of flower and aerial parts showed MIC as 0.15 and 1.25 mg/ml respectively against Candida albicans. In conclusion, the S. indicus flower n-hexane extract seems to be a promising antimicrobial agent.
Duraipandiyan, V.; Kannan, P.; and Ignacimuthu, S.
"Antimicrobial Activity of Sphaeranthus indicus L.,"
2, Article 5.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2009/iss2/5