Seedlings of Glycine max L. were grown, transplanted and acclimatized for 60 days at different altitudes (250, 400 and 1600 m). Response to shift in altitude was observed in the plants. Shoot length decreased with the increase in the altitude, while root length followed a reverse trend. Biomass accumulation in shoot and roots of G. max was the maximum at high altitude. Total soluble protein content was significantly high at low altitude in the shoot and the roots. Free tissue ammonia level in this species showed positive correlation with increasing altitude. Ammonium assimilatory enzymes viz., glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) were studied. GS/ GOGAT specific and total activity were altitude sensitive, whereas GDH activity exhibited inverse trend. Results indicate that there is a positive shift in ammonium assimilatory pathway in plants growing at high altitude.
Rajasekaran, C.; Kalaivani, T.; Jayakumararaj, R.; Singh, Abhijeet; Pusalkar, V. R.; and Marimuthu, R.
"Studies on the Impact of Altitudinal Gradient on Ammonium Assimilatory Metabolism in Glycine max L. (Fabaceae),"
Ethnobotanical Leaflets: Vol. 2009
, Article 2.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2009/iss2/2