The compatibility of Metarhizium anisopliae with Ocimum sanctum was studied in vitro. Leaves, roots, stems and seed extracts of O. sanctum were mixed in a Potato Dextrose Agar and Potato Dextrose Broth. M. anisopliae was inoculated and the mycelial dry weight and spore count were assessed. The behavior of the fungus with the extracts was similar in terms of mycelial dry weight, except for methanol extracts of leaves, ether extracts of roots, water and acetone extracts of seeds and benzene, methanol and acetone extracts of stems which reduced the mycelial dry weight of the fungal colonies. Benzene extract of leaves and methanol extract of roots of O. sanctum were found to be highly compatible with M. anisopliae whereas ether extract of roots and benzene as well as acetone extracts of stem were classified as very toxic. The results of the current study revealed that O. sanctum extracts did not affect the inoculum potential of M. anisopliae in terms of mycelial dry weight and spore count and hence M. anisopliae was compatible with O. sanctum.
Borgio, J. Francis; Bency, B. Jesvin; and Sharma, Neha
"Compatibility of Metarhizium anisopliae (Metsch.) Sorok. with Ocimum sanctum Linn. (Tulsi) (Lamiaceae) Extracts,"
Ethnobotanical Leaflets: Vol. 2008
, Article 94.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2008/iss1/94