The present study optimized the regeneration protocol by using half seed explant in Vigna mungo (L.) Hepper organogenesis. Half seed explants were inoculated onto B5 medium supplemented with kinetin (4.7 μM to 23.5 μM), 6- benzyladenine (4.4 μM to 22.2 μM), naphthaleneacetic acid (5.4 μM to 27.0 μM), indolebutyric acid (4.9 μM to 24.5 μM) and 2,4- dichlorophenoxyacetic acid (4.5 μM to 22.5 μM). Callus initiation was observed in all media evaluated and the highest cell proliferation was obtained from explants cultivated in the presence of 13.3 μM BAP and 13.5 μM 2,4-D. Shoot induction was obtained from callus induced on 13.3 μM BAP and 13.5 μ 2,4-D at 6 weeks after transferring the callus to a B5 medium supplemented with 13.3 μM BAP. Roots were induced from shoots on B5 media with indolebutyric acid (IBA, 14.7 μM) and then regenerated plants were hardened and acclimated in greenhouse conditions.
Harisaranraj, R.; Babu, S. Saravana; and Suresh, K.
"Callus Induction and Plant Regeneration of Vigna mungo (L.) Hepper via Half Seed Explant,"
Ethnobotanical Leaflets: Vol. 2008
, Article 76.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2008/iss1/76