The present study describes a protocol for rapid and large scale in vitro micropropagation of Talinum portulacifolium L. through axillary bud culture. The culture medium was optimized for propagation and high ex vitro survival rate was achieved. MS medium supplemented with 6µM BAP and 2µM IAA in combination was shown a better efficiency of shoot proliferation. Maximum of 8 new shoots were developed from a single explant (axillary bud) after 3 subcultures (subculture interval 15 days). Root development was facilitated by MS medium supplemented with both IBA (4µM) and NAA(1µM). Well rooted and partially acclimatized plantlets were transferred into poly cups for further acclimation.
Thangavel et al., K.
"In Vitro Micropropagation of Talinum portulacifolium L. through Axillary Bud Culture,"
Ethnobotanical Leaflets: Vol. 2008
, Article 49.
Available at: http://opensiuc.lib.siu.edu/ebl/vol2008/iss1/49